... Restriction Endonucleases (sometimes just called Restriction Enzymes or RE s). These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs...
...from E. Coli bacteria--is able to recognize the base sequence 5 GAATTC 3 . Restriction enzymes cut each strand of DNA between the G and the A in this sequence ...
...限制酶) cuts at a restriction site, which is generally palindromic (because restriction enzymes often works as homodimers). Among all the sequences listed, only GGCC is palindromic, which means they ...
only 5 point?? http://www.neb.com/nebecomm/tech_reference/ restriction _ enzymes /dna_ sequences _maps.asp? 找找看有沒你要的吧 有pdf檔 或如果你有 sequence 的話可...
restriction enzyme 會認palindrome的序列也就是這段必須兩股的5 ->3 讀起來一樣答案只有3因為它...
...以上的位置來切DNA,切DNA的同時也會將自己摧毀 type II restriction enzymes :是一群相當特殊的DNA內切脢會辨認出DNA上的特定辨識序列(recognition sequences )。 常用限制脢的辨識序列為雙股DNA上呈迴文結構的4~8個鹼基對。有兩...
...連接到資料庫,下載更多你需要的plasmid sequence 。要畫MAP,只要把序列弄好,勾選你要顯示的 enzyme site,他自動會幫你畫好。這套軟體...限制酶圖譜的程式是準備用來畫plasmid的 restriction enzyme site之map嗎? 2006-03-12 21:28...
1.溶解ssDNA用TE buffer是一般比較常見的,只要不是要接著做 restriction enzyme digestion都很合適 至於TE buffer的濃度與pH值,一般而言用一倍...
...矮小侏儒的生長等。 進行重組DNA需要的工具 1. 限制性內切( restriction enzymes ) 限制性內切酵素會認出特定序列(recognition sequences ),此特定序列為雙股DNA上4-8個核甘酸,有相同的序列,但...